Library Prep Method Melds DNA Quantification and Size Selection into Single Step with Digital PCR | GenomeWeb
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DNA Size Selection System 1 ChIP - Seq Library Prep Application Note 02
One of the technically challenging and labor-intensive steps is the size selection of DNA fragments during the NGS library construction and is a source of sample loss and sample cross contamination. This tech note is a comparison of two alternative methods for the size-selection step: Pippin PrepTM automated DNA sizing and E-gel® SizeSelectTM gels, for the purpose of determining the suitability...
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Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...
متن کاملChallenges to Design and Develop of DNA Aptamers for Protein Targets. I. Optimization of Asymmetric PCR for Generation of a Single Stranded DNA Library
Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of ssDNA. The purpose of this study was to design a random DNA library for selection of aptamers wit...
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Due to the high cost of failed runs and suboptimal data yields, quantification and determination of fragment size range are crucial steps in the library preparation process for massively parallel sequencing (or next-generation sequencing). Current library quality control methods commonly involve quantification using real-time quantitative PCR and size determination using gel or capillary electr...
متن کاملLow input DNA size selection on the Pippin Prep System using the Agilent 2100 Bioanalyzer with the Agilent High Sensitivity DNA kit
With the advent of next-generation sequencing (NGS), library preparation has become a much more streamlined process utilizing smaller amounts of input genomic DNA, thus allowing for more narrow fragmentation profi les. This Application Note demonstrates the usefulness of the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA kit in complementing the Pippin Prep automated size selecti...
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